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This Question 6- Download a molecule from the Protein Data Bank (PDB) in pdb format. Prepare a chime enhanced web page showing this molecule in two different display formats. Include enough information about this molecule in the web page, so that others would be able to understand this structure. The length of this web page should not be less than two pages
 

 






 

This pathway was contributed by Xiao-yun Zhang and Stephen Stephens, University of Minnesota.

Acrylonitrile is microbially metabolized by Pseudomonas chlororaphis to acrylamide, a building block for various plastic polymers. Over 200,000 tons of acrylamide are produced annually in this way, 90% of which is used in the production of polyacrylamide polymers. These polymers are used for water treatment, storage, distribution and in polyacrylamide gels used for macromolecular analysis (Glazer, A.W. and Nikaido, H. Microbial Biotechnology. New York: W.H. Freeman, 1995).

The following is a text-format acrylonitrile degradation pathway map. An organism which can initiate the pathway is given, but other organisms may also carry out later steps. Follow the links for more information on compounds or reactions. This map is also available in graphic (12k) format.

       Acrylonitrile                    Acrylonitrile
    Pseudomonas chlororaphis
            k22                            strain B23 
             |                                |
             |                                |
             |                                | nitrile hydratase
             |                                |
             |                                |
             | aliphatic                      v
             | nitrilase                  Acrylamide      
             |                                |
             |                                |
             |                                | amidase
             |                                |
             |                                |
             |                                v            from the
             +------------------------->  Acrylate <------ Styrene Pathway
                                              |
                                              |
                                              | glutaconate CoA-transferase
                                              |
                                              |
                                              v
                                          Acrylyl-CoA
                                              |
                                              |
                                              | lactoyl-CoA dehydratase
                                              |
                                              |
                                              v
                                         Lactoyl-CoA
                                              |
                                              |
                                              | propionate CoA-transferase
                                              |
                                              |
                                              v
                                          L-Lactate
                                              |
                                              |
                                              |
                                              |
                                              |
                                              v 
                                 Intermediary Metabolism (KEGG)
QUESTION 5